|
Table 1.
Available P, total P, kjhel N, exchanglable K (ppm) and % organic
carbon in the soil samples collected from Krishi Tirth, Bajwada, Dewas
(MP). |
|
Treatment |
Available P |
Total P |
Kjehldahl
(organic form) N |
Exchangable
(available) K |
%OC |
pH
|
|
(ppm) |
(ppm) |
(ppm) |
(ppm) |
(ppm) |
|
Original Soil |
17.1 |
392 |
174 |
284 |
0.66 |
7.75 |
|
Between Heaps |
20.5 |
362 |
198 |
315 |
0.74 |
7.59 |
|
Planted Heap |
33.1 |
410 |
194 |
424 |
0.72 |
7.91 |
|
Below heap |
247.7 |
500 |
798 |
770 |
2.61 |
7.89 |
|
|
|
|
|
|
|
|
|
Mean |
79.6 |
416 |
341 |
448.25 |
1.1825 |
7.79 |
|
SE+ |
17.7*** |
58.4NS |
77.8** |
87.0* |
0.264*** |
0.036*** |
|
*= Differences
across treatments are statistically significant at probability level (P)
0.05 **=Differences across treatments are statistically significant
at P 0. 01; ***= Differences across treatments are
statistically significant at P 0.001, NS= Differences across
treatments are statistically non significant |
|
Soil sampled on
19.09.07. |
|
Original soil =
soil sample from unplanted area on the farm |
|
Between Heaps =
Planting concept on the farm is grow horticultural crops on heaps and
heaps are widely apart, soil sampling in this treatment was done
between heaps. |
|
Planted Heap =
Sampling in this treatment was done at the heap, besides a growing
plant on top of a heap |
|
Below Heap =
Sampling in this treatment was done after removing all the soil and
plant roots from soil surface. Sampling was done from area just below
the soil surface but below the heap. |
|
Replications:
each of the four treatments had three replications, and there were
about three spots within a given replications. |
|
On the
different parameters that were measured:
A plant needs
over 30 different elements for its growth/formation of leaves, stem
fruits etc. all body parts. But we generally measure only selected few
and largely nitrogen (N), phosphorus (P) and potash (K). All
the 30 about elements occur in a soil largely in two forms –
‘available’ and ‘non-available’ form. Wherever it is stated as
‘Total’ it means it is total of available plus non-available form. The
available form of a nutrient can be readily taken-up by a plant
through its roots while the other form has to be processed by
microorganisms, which are in maximum numbers on surface of roots and
convert them into available form, through enzyme activities or
production of organic acids. The process of conversion will generally
be slow and would depend on type and numbers of different
microorganisms. An element provided as a ‘fertilizer’ is essentially
in available form and therefore when applied to soil, we generally
notice a rapid response of plants, in terms of increased green color
of foliage and/or growth/yield. pH tells us whether a soil is close to
normal or a problematic soil. For a very good soil, pH should be
around 7, and values more than 8 (salinity/alkalinity) and less than 6
(acidity) indicate problem. Note: All these elements
come from mother rock from which a soil has formed. Formation of soil
is very long process. Few centimeters layer of soil might have taken
thousands of years to get formed.
Organic carbon (OC)
is a biological and not a chemical parameter. Unlike the other
biological parameters, this can be measured readily by a chemistry
laboratory and is therefore generally lumped with the chemical
parameters. OC% is like a bank of nutrients in soil and may
contain all the nutrients needed for plant growth. More the value,
bigger will be the bank balance. But like other elements in soil, much
of these elements are also in unavailable form for a plant, but
relatively easily degradable to become available for use by plants.
These can be made available to plants by microbial activity and carbon
in this component serves as food for the microorganisms.
Comments on data table 1:
(a) fertility of the original soil was lower than the area under
cultivation; (b) fertility was maximum below (15 cm) the heap
indicating that roots from plants sown on heaps will tend to go deep
in the soil to explore/take-up the nutrients; (c) organic carbon
percent (OC%) below heap was at least 3 times more than that in the
heap itself, indicating that smaller carbonaceous molecules of
degrading biomass move down from heaps with water (rain or
irrigation). |
|
Table 2.
Total B, S, Fe, Zn (ppm), and Available B, S, Fe, Zn and Mo (ppm), in
the soil samples collected from Bajwada (MP), sampled on 19.09.07.
|
|
|
Total B |
Available B |
Total S |
Available S |
Total Fe |
Available Fe |
Total Zn |
Available Zn |
Available Mo |
|
Treatment |
(ppm) |
(ppm) |
(ppm) |
(ppm) |
(ppm) |
(ppm) |
(ppm) |
(DTPA-Zn) |
(ppm) |
|
Original Soil |
29.7 |
0.27 |
93 |
7.17 |
40442 |
15.6 |
133 |
0.83 |
0.019 |
|
Between Heap |
26.0 |
0.29 |
103 |
7.00 |
33550 |
11.7 |
108 |
1.08 |
0.009 |
|
Planted Heap |
27.0 |
0.32 |
94 |
7.60 |
34625 |
9.1 |
77 |
0.97 |
0.012 |
|
Below heap |
26.7 |
2.29 |
420 |
18.93 |
33300 |
21.0 |
97 |
6.10 |
0.020 |
|
|
|
|
|
|
|
|
|
|
|
|
Mean |
27.3 |
0.79 |
178 |
10.18 |
35479 |
14.4 |
104 |
2.25 |
0.015 |
|
SE+ |
1.11NS |
0.215*** |
21.0*** |
1.054*** |
1641.1NS(0.06) |
2.48* |
21.7NS |
0.293*** |
0.0031NS |
|
CV% |
7 |
47 |
21 |
18 |
8 |
30 |
36 |
23 |
37 |
|
* =
Statistically significant at 0.05, *** = Statistically significant at
0.001, NS= Statistically non-significant |
|
NS (0.06) =
Statistically nonsignificant at p=0.05 but the values are
statistically significantly different at p=0.06. |
|
On the different parameters that
were measured:
As stated above,
a plant needs over 30 different elements for its growth and good yield
and these should be in balanced form. The three elements nitrogen (N),
phosphorus (P) and potash (K) are called major elements because these
are required in relatively large quantities compared to the others.
Ten other elements [B
(boron), Ca (calcium), Mg (magnesium), S (sulphur), Fe (iron), Mn
(manganese) , Mo (molybdenum), Cu (copper), Zn (zinc) and Cl
(chloride)]
are regarded as vital elements for plant growth along with the P and
K. These ten are widely known as micro-elements because these are
needed in micro quantities - parts per million (ppm). Like the major
elements these also occur in
‘available’ and
‘non-available’
form. As stated
above, an agricultural field would highly likely have all the over 30
elements needed for crop growth, but they would largely be in
‘unavailable’ form. But interestingly, much of the soil analyses done
by scientific community is only for the ‘available’ form and not for
the total amount of any given element in the soil. Also, it is worth
noting that all the recommendations of a given fertilizer by the
extension agencies or by fertilizer dealers is based on the available
quantity of an element.
Note:
For good crop growth, other 18 elements are also needed, but in very
miniscule quantities and these are regarded as ‘Trace Elements’.
Comments on
data table 2:
Only five of the ten micro-nutrients, widely noted as deficient in
farmers’ fields in semi-arid tropics [see paper by Sahrawat et al.
2007; Current Science 93(10):1428-1432], were analyzed. Salient
comments follow: (a) quantities of available form of nutrients (B, S,
Fe, Mo and Zn) were invariably significantly more below the heap than
that at other sampling spots of the same field; (b) total
concentration of all these elements was similar across sampling spots
except for ‘total S’ indicating addition of ‘S’ with the items such as
‘Amrit pani etc. being applied, and this needs to be studied; (c) the
noted small differences across sampling spots in the total
concentration of three elements -- B, Fe and Z were statistically
non-significant. It was apparent that the heap method of cultivation
has ability to continuously converting insoluble form of nutrients to
soluble form (note: heap remains moist due to continuous application
of water) and therefore potentially obviates the need of dependence on
market purchased elements. Discussion with soil scientists indicated
that most soils would have total form of most elements. |
|
Table 3.
Biomass carbon, biomass nitrogen and dehydrogenase activity in the
soil samples collected from Krishi Tirth, Bajwada, Dewas (MP) |
|
|
|
|
|
|
Treatment |
Microbial
Biomass C |
Microbial
Biomass N |
Dehydrogenase
activity |
|
Original Soil |
376 |
37 |
58 |
|
Between Heap |
274 |
33 |
38 |
|
Planted Heap |
208 |
34 |
63 |
|
Below heap |
426 |
66 |
98 |
|
|
|
|
|
|
Mean |
321 |
42 |
64 |
|
SE+ |
79.8NS |
19.3NS |
26.9NS |
|
|
|
|
|
|
NS= Differences
across treatments are statistically non-significant |
|
|
|
On the
different parameters measured:
Microbial
biomass carbon:
this parameter tells us about the carbon held in body of
microorganisms, and is an indirect measure of total population of
microorganisms, irrespective of their culturability. Note:
microbiologists can only culture (in laboratory conditions) about 10%
of microbial life in a given niche – a generalization. But this does
not mean that the un-culture-able microorganisms are not functioning
in nature. It only means that we do not fully understand their
importance/value.
Microbial
biomass nitrogen:
this parameter tells us about the nitrogen held in the body of
microorganisms, an indirect parameter of total population of
microorganisms, irrespective of their culturability.
Dehydrogenase
activity:
like the above two parameters, this also reflects all microbial life
in a given niche, irrespective of culturability limitations. This
reflection is recorded through activity of this enzyme having over 10
sub-types by oxidizing several different substrates of the several
biochemical processes operating inside a living microorganism.
Comments on data table 3:
(a)
as indicated by microbial biomass carbon and nitrogen, the soil below
heaps had most microbial activity/population followed by that in the
original soil while the activity in the decomposing biomass in heaps
was next highest, (b) activity of microorganisms as indicated by
‘dehydrogenase’ enzyme was also maximum in the sample collected below
the heaps, followed by that in the heap itself, and lowest activity
was noted in unplanted area between heaps which was covered with dry
biomass, the noted high activity in the original soil is perhaps due
to good growth of grass that would have allowed a good level of
microbial activity in its root rhizosphere and needs further
consideration. |
|
Table 4:
Population (log10 g-1 dry soil) and diversity (no.
of colonies of different types) of different microorganisms
in
the soil samples collected from Krishi Tirth, Bajwada, Dewas (MP) |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Treatment |
Total bacteria
pop. |
Total bacteria
diversity |
Actinomycetes pop. |
Actinomycetes
diversity |
Fungal pop. |
Fungal diversity |
Plant growth
promoters (Ab) |
Pseudomonas
pop.,suppress diseases
(Ab) |
P-solubilizers
pop. (Ab) |
Org. Acid
producers pop. (Ab) |
N2-fixers,
AZO like pop (Ab). |
|
Original |
6.64 |
5 |
5.67 |
8 |
4.02 |
6 |
4.94 |
<4.0 |
<3.0 |
3.33 |
4.33 |
|
Between heaps |
6.80 |
7 |
5.30 |
6 |
4.34 |
6 |
4.77 |
<4.0 |
<3.0 |
3.67 |
4.09 |
|
Planted heap |
7.20 |
7 |
5.67 |
5 |
4.51 |
3 |
5.57 |
<4.0 |
<3.0 |
5.33 |
4.28 |
|
Below heap |
6.86 |
11 |
5.58 |
7 |
4.18 |
6 |
3.85 |
<4.0 |
<3.0 |
4.00 |
4.16 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Mean |
6.87 |
8 |
5.55 |
6 |
4.26 |
5 |
4.79 |
<4.0 |
<3.0 |
4.08 |
4.22 |
|
SE+ |
0.099* |
0.6*** |
0.103NS |
0.9NS |
0.152NS |
1.4NS |
0.482NS |
<4.0a |
<3.0a |
1.244NS |
0.135NS |
|
a=Population of
Pseudomonas spp. and P-solubilizers could not be assessed due to
presence of large numbers of other bacteria. |
|
*= Differences
across treatments are statistically significant at probability level (P)
0.05 **=Differences across treatments are statistically significant at
P 0. 01 ***= Differences across treatments
are statistically significant at P 0.001 NS= Differences across
treatments are statistically non significant |
|
On the
different parameters measured:
Total population
of bacteria, actinomycetes and fungi:
this parameter tells us about the population of these types of
microorganisms that can grow on selected recipes (different for
different microorganisms) where microbiologists believe that majority
microorganisms will grow. It may, however, be noted that microbiologists
can culture about 10% of total population of microorganisms in any
niche, due to limitations of methods of culturing. Note:
all populations are log numbers and have to be taken accordingly. For
example, log 3 means 1000 and log 6 means 10 lakh.
Diversity of
bacteria, actinomycetes, and fungi :
this tells us the different types (due to size, color, texture etc. of
the microbial colony) of microorganisms noted on the growth medium
(recipes) used for population count (above parameter). Thus it does not
account for the total microbial diversity in a given niche.
Agriculturally
beneficial bacteria (Ab):
All the five
parameters (last five columns) indicated by (Ab) [the last five columns
of this table] are the five different functional group of bacteria with
functions as indicated with their names.
Comments on data table 4:
(a)
Population of bacteria inside heaps and below heaps was significantly
more than the other treatments (range from 6.64 to 6.80 log10
per g of soil); (b) population of actinomycetes and fungi was similar
across the four treatments and ranged from 5.30 to 5.67 ( log10
per g of soil) in case of actinomycetes and from 4.00 to 4.51 (log10
per g of soil) in case of fungi; (c) maximum population of the plant
growth promoters and organic acid producers was inside heaps where lot
of roots were noted during sampling and lowest in the soil below the
heaps where chemical fertility was the highest; (d) population of
Psuedomonas (indicators of ability of soil to manage diseases) and
P-solubilizers could not be counted due to methodology problems; (e) N2-fixing
bacteria (colonies that were looking like Azotobacter) was
similar across the four treatments. |
|